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DOTMA is a cationic lipid employed as a non-viral vector in gene therapy. As a component of liposomes, DOTMA is used to encapsulate siRNA, microRNA, and oligonucleotides, and is applied in vitro for gene transfection. By inducing a positive charge on the liposomes, DOTMA facilitates effective interaction between the liposomes and cell membranes. DOTMA demonstrates commendable gene transfection efficiency both in vitro and in vivo.